The "Mechanisms of Assemblies Formation Essential to Muscle Function" project's collaborative team united by HFSP Award will capitalise on complementary and synergistic expertise in structural biology and modelling (Kristina Djinovic Carugo, EMBL Grenoble, France - https://www.embl.org/groups/djinovic/ and Toshiyuki Oda, University of Tokyo, Japan), advanced imaging (Jonas Ries, University of Vienna, Austria - https://www.maxperutzlabs.ac.at/research/research-groups/ries) and cardiovascular development and disease (Travis Hinson, The Jackson Laboratory, Farmington, CT, USA - https://www.jax.org/research-and-faculty/research-labs/the-hinson-lab) and adopt a multifaceted and multiscale approach to tackle the central questions on sarcomere cytoskeleton assembly in biogenesis at the molecular and structural level.